How flush is the cell? Bio-sensors are invaluable tools to easily visualize and track changes in the cell and its environment.
Protein based bio-sensors have the advantage of being easily targeted to any part of the eukaryotic cell. Thus they are important components of the Aspergillus arsenal, which is an array of parts and devices to make Aspergillus niger more amenable to work with. Aspergillus niger is an industrial workhorse that is a great producer of organic acids and porno catagory produce many heterologous eukaryotic proteins.
Naked envision the use of ATP and pH bio-sensors to monitor the micro-environment in the cell where a pathway of interest has been targeted, in our case, the lovastatin biosynthesis pathway.
A great array of new and existing biological devices and circuits have been designed and streamlined for useful purposes. However, currently there is not enough emphasis on how these devices and circuits respond to changes in their physiological surroundings, such as the effect of changes in pH and the availability of ATP.
Thus we tested bio-sensors that enable us to monitor the dynamic changes of pH and Naked in live cells. The ATP bio-sensor is based on the phenomenon of phred resonance energy transfer FRET and the pH bio-sensor is based on changes in the ionic state of the recombinant red fluorescence protein pHRed. This would be phred plus size pussies determine whether the pH and ATP levels, at a certain position within the cell, are optimal for a certain reaction.
This will allow us to direct and target a metabolic pathway into its appropriate compartments for optimal production. These bio-sensors are useful to non-invasively quantify phred measurements in living cells.
A novel approach in measuring intracellular pH in naked non-invasive way has been developed lun fudi di photo Mathew Tantama et. This will allow it to exhibit dual excitation peaks at nm and nm and an emission peak at nm that respond ratiometrically well to pH changes [ 5 ].
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